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A curated list of awesome Bioinformatics libraries and software.


danielecookdanielecook/Awesome-BioinformaticsUpdated 625 83 164

Awesome Bioinformatics Awesome Build Status

Bioinformatics is an interdisciplinary field that develops methods and software tools for understanding biological data. — Wikipedia

A curated list of awesome Bioinformatics software, resources, and libraries. Mostly command line based, and free or open-source. Please feel free to contribute!

Table of Contents

Package suites

Package suites gather software packages and installation tools for specific languages or platforms. We have some for bioinformatics software.


  • Bioconductor - A plethora of tools for analysis and comprehension of high-throughput genomic data, including 1500+ software packages.


  • Biopython - Freely available tools for biological computing in Python, with included cookbook, packaging and thorough documentation. Part of the Open Bioinformatics Foundation. Contains the very useful Entrez package for API access to the NCBI databases.


  • Bioconda - A channel for the conda package manager specializing in bioinformatics software. Includes a repository with 3000+ ready-to-install (with conda install) bioinformatics packages.

Data Processing

Command Line Utilities

  • Bioinformatics One Liners - Git repo of useful single line commands.
  • BioNode - Modular and universal bioinformatics, Bionode provides pipeable UNIX command line tools and JavaScript APIs for bioinformatics analysis workflows.
  • bioSyntax - Syntax Highlighting for Computational Biology file formats (SAM, VCF, GTF, FASTA, PDB, etc...) in vim/less/gedit/sublime.
  • CSVKit - Utilities for working with CSV/Tab-delimited files.
  • csvtk - Another cross-platform, efficient, practical and pretty CSV/TSV toolkit.
  • datamash - Data transformations and statistics.
  • easy_qsub - Easily submitting PBS jobs with script template. Multiple input files supported.
  • GNU parallel - General parallelizer that runs jobs in parallel on a single multi-core machine. Here are some example scripts using GNU parallel.
  • grabix - A wee tool for random access into BGZF files.
  • tabix - Table file index.
  • wormtable - Write-once-read-many table for large datasets.
  • zindex - Create an index on a compressed text file.

Next Generation Sequencing

Pipelines/Pipeline frameworks

  • Awesome-Pipeline - A list of pipeline resources.
  • bcbio-nextgen - Batteries included genomic analysis pipeline for variant and RNA-Seq analysis, structural variant calling, annotation, and prediction.
  • BigDataScript - A cross-system scripting language for working with big data pipelines in computer systems of different sizes and capabilities.
  • Bpipe - A small language for defining pipeline stages and linking them together to make pipelines.
  • Common Workflow Language - a specification for describing analysis workflows and tools that are portable and scalable across a variety of software and hardware environments, from workstations to cluster, cloud, and high performance computing (HPC) environments.
  • Cromwell - A Workflow Management System geared towards scientific workflows.
  • Galaxy - a popular open-source, web-based platform for data intensive biomedical research. Has several features, from data analysis to workflow management to visualization tools.
  • GATK Queue - A pipelining system built to work natively with GATK as well as other high-throughput sequence analysis software.
  • Nextflow - A fluent DSL modelled around the UNIX pipe concept, that simplifies writing parallel and scalable pipelines in a portable manner.
  • R-Peridot - Customizable pipeline for differential expression analysis with an intuitive GUI.
  • Ruffus - Computation Pipeline library for python widely used in science and bioinformatics.
  • SeqWare - Hadoop Oozie-based workflow system focused on genomics data analysis in cloud environments.
  • Snakemake - A workflow management system in Python that aims to reduce the complexity of creating workflows by providing a fast and comfortable execution environment.
  • Workflow Descriptor Language - Workflow standard developed by the Broad.

Sequence Processing

Sequence Processing includes tasks such as demultiplexing raw read data, and trimming low quality bases.

  • AfterQC - Automatic Filtering, Trimming, Error Removing and Quality Control for fastq data
  • FastQC - A quality control tool for high throughput sequence data.
  • Fastqp - FASTQ and SAM quality control using Python.
  • Fastx Tookit - FASTQ/A short-reads pre-processing tools: Demultiplexing, trimming, clipping, quality filtering, and masking utilities.
  • MultiQC - Aggregate results from bioinformatics analyses across many samples into a single report.
  • SeqKit - A cross-platform and ultrafast toolkit for FASTA/Q file manipulation in Golang.
  • seqmagick - file format conversion in Biopython in a convenient way
  • Seqtk - Toolkit for processing sequences in FASTA/Q formats.
  • smof - UNIX-style FASTA manipulation tools.

Sequence Alignment

De Novo Alignment

DNA Resequencing

  • Bowtie 2 - An ultrafast and memory-efficient tool for aligning sequencing reads to long reference sequences.
  • BWA - Burrow-Wheeler Aligner for pairwise alignment between DNA sequences.

Variant Calling

  • freebayes - Bayesian haplotype-based polymorphism discovery and genotyping.
  • GATK - Variant Discovery in High-Throughput Sequencing Data.
  • samtools/bcftools/htslib - A suite of tools for manipulating next-generation sequencing data.

BAM File Utilities

  • Bamtools - Collection of tools for working with BAM files.
  • bam toolbox MtDNA:Nuclear Coverage; BAM Toolbox can output the ratio of MtDNA:nuclear coverage, a proxy for mitochondrial content.
  • mergesam - Automate common SAM & BAM conversions.
  • SAMstat - Displaying sequence statistics for next-generation sequencing.
  • Telseq - Telseq is a tool for estimating telomere length from whole genome sequence data.

VCF File Utilities

  • bcftools - Set of tools for manipulating VCF files.
  • vcfanno - Annotate a VCF with other VCFs/BEDs/tabixed files.
  • vcflib - A C++ library for parsing and manipulating VCF files.
  • vcftools - VCF manipulation and statistics (e.g. linkage disequilibrium, allele frequency, Fst).

GFF BED File Utilities

  • gffutils - GFF and GTF file manipulation and interconversion.
  • BEDOPS - The fast, highly scalable and easily-parallelizable genome analysis toolkit.
  • Bedtools2 - A Swiss Army knife for genome arithmetic.

Variant Simulation

  • Bam Surgeon - Tools for adding mutations to existing .bam files, used for testing mutation callers.
  • wgsim - Comes with samtools! - Reads simulator.

Variant Filtering / Quality Control

Variant Prediction/Annotation

  • SIFT - Predicts whether an amino acid substitution affects protein function.
  • SnpEff - Genetic variant annotation and effect prediction toolbox.

Python Modules


  • cruzdb - Pythonic access to the UCSC Genome database.
  • pyensembl - Pythonic Access to the Ensembl database.
  • bioservices - Access to Biological Web Services from Python.



Genome Browsers / Gene Diagrams

The following tools can be used to visualize genomic data or for constructing customized visualizations of genomic data including sequence data from DNA-Seq, RNA-Seq, and ChIP-Seq, variants, and more.

  • Squiggle - Easy-to-use DNA sequence visualization tool that turns FASTA files into browser-based visualizations.
  • biodalliance - Embeddable genome viewer. Integration data from a wide variety of sources, and can load data directly from popular genomics file formats including bigWig, BAM, and VCF.
  • BioJS - BioJS is a library of over hundred JavaScript components enabling you to visualize and process data using current web technologies.
  • Circleator - Flexible circular visualization of genome-associated data with BioPerl and SVG.
  • DNAism - Horizon chart D3-based JavaScript library for DNA data.
  • IGV js - Java-based browser. Fast, efficient, scalable visualization tool for genomics data and annotations. Handles a large variety of formats.
  • Island Plot - D3 JavaScript based genome viewer. Constructs SVGs.
  • JBrowse - JavaScript genome browser that is highly customizable via plugins and track customizations
  • PHAT - Point and click, cross platform suite for analysing and visualizing next-generation sequencing datasets.
  • pileup.js - JavaScript library that can be used to generate interactive and highly customizable web-based genome browsers.
  • scribl - JavaScript library for drawing canvas-based gene diagrams. The Homepage has examples.

Circos Related

  • Circos - Perl package for circular plots, which are well suited for genomic rearrangements.
  • ClicO FS - An interactive web-based service of Circos.
  • OmicCircos - R package for circular plots for omics data.
  • J-Circos - A Java application for doing interactive work with circos plots.
  • rCircos - R package for circular plots.

Database Access


Becoming a Bioinformatician


  • Next-Generation Sequencing Technologies - Elaine Mardis (2014) [1:34:35] - Excellent (technical) overview of next-generation and third-generation sequencing technologies, along with some applications in cancer research.
  • Annotated bibliography of *Seq assays - List of ~100 papers on various sequencing technologies and assays ranging from transcription to transposable element discovery.
  • For all you seq... (PDF) (3456x5471) - Massive infographic by Illumina on illustrating how many sequencing techniques work. Techniques cover protein-protein interactions, RNA transcription, RNA-protein interactions, RNA low-level detection, RNA modifications, RNA structure, DNA rearrangements and markers, DNA low-level detection, epigenetics, and DNA-protein interactions. References included.



YouTube Channels and Playlists

  • Current Topics in Genome Analysis 2016 - Excellent series of fourteen lectures given at NIH about current topics in genomics ranging from sequence analysis, to sequencing technologies, and even more translational topics such as genomic medicine.
  • GenomeTV - "GenomeTV is NHGRI's collection of official video resources from lectures, to news documentaries, to full video collections of meetings that tackle the research, issues and clinical applications of genomic research."
  • Leading Strand - Keynote lectures from Cold Spring Harbor Laboratory (CSHL) Meetings. More on The Leading Strand.
  • Genomics, Big Data and Medicine Seminar Series - "Our seminars are dedicated to the critical intersection of GBM, delving into 'bleeding edge' technology and approaches that will deeply shape the future."
  • Rafael Irizarry's Channel - Dr. Rafael Irizarry's lectures and academic talks on statistics for genomics.
  • NIH VideoCasting and Podcasting - "NIH VideoCast broadcasts seminars, conferences and meetings live to a world-wide audience over the Internet as a real-time streaming video." Not exclusively genomics and bioinformatics video but many great talks on domain specific use of bioinformatics and genomics.


  • ACGT - Dr. Keith Bradnam writes about this "thoughts on biology, genomics, and the ongoing threat to humanity from the bogus use of bioinformatics acroynums."
  • Opiniomics - Dr. Mick Watson write on bioinformatics, genomes, and biology.
  • Bits of DNA - Dr. Lior Pachter writes review and commentary on computational biology.
  • it is NOT junk - Dr. Michael Eisen writes "a blog about genomes, DNA, evolution, open science, baseball and other important things"




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